How To Calculate Enrichment Score Gene Ontology Enrichment Analysis

Introduction & Importance

Gene Ontology (GO) Enrichment Analysis is a crucial step in understanding the biological significance of a list of genes. It helps identify which GO terms (biological processes, cellular components, or molecular functions) are overrepresented in your gene list compared to a reference genome.

How to Use This Calculator

1. Enter your gene list, separated by commas.
2. Select the ontology you’re interested in.
3. Enter the p-value threshold.
4. Click ‘Calculate’.

Formula & Methodology

The enrichment score is calculated using the hypergeometric distribution. The formula is…

Real-World Examples

Data & Statistics

Example GO Terms and P-values

GO Term	P-value
GO:0006810 – DNA replication	0.001

Expert Tips

• Consider using a multiple testing correction method like Benjamini-Hochberg to control the false discovery rate.
• Interpret the results in the context of your biological question and experimental design.

Interactive FAQ

What is the difference between Biological Process, Cellular Component, and Molecular Function?

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NCBI’s Guide to GO and QuickGO are excellent resources for understanding GO.