Seahorse Mito Stress Test Calculations
Seahorse mito stress test calculations are crucial for understanding mitochondrial function in cells. This calculator helps you perform these calculations accurately.
- Enter the Basal OCR, Maximal OCR, and Adenosine 5′-O-Diphosphate (AO) values.
- Click ‘Calculate’.
- View your results and chart below.
The calculation involves finding the ratio of maximal OCR to basal OCR, and then adjusting for AO levels.
| Case | Basal OCR | Maximal OCR | AO | Result |
|---|---|---|---|---|
| 1 | 50 | 200 | 2 | 3.0 |
| Parameter | Value |
|---|---|
| Average Basal OCR | 60 |
- Always use fresh reagents for accurate results.
- Ensure your samples are properly prepared to avoid contamination.
What is the difference between Basal OCR and Maximal OCR?
Basal OCR is the oxygen consumption rate of cells in their resting state, while maximal OCR is the rate after uncoupling agents have been added to stimulate maximal respiration.